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In this study we have shown how a purine nucleoside triphosphate, Guanosine triphosphate (GTP), binding establishes rigidity-based allosteric communication with the regulatory coenzyme NADH and then synergistically inhibits the oxidative deamination activity of mammalian GDH by stabilizing its closed conformation
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According to the effects we observed in our murine in vivo experiments, immunoblotting of IHKE-1 cells also demonstrated upregulation of HSP70 and p-Bad and downregulation of p14-3-3ζ upon glutamine treatment in vitro (Supplemental Figure 6, B–E)
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