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Cells were harvested by centrifugation and resuspended in lysis buffer (20 mM Tris-HCl, pH 8.0, 300 mM NaCl, 5% glycerol)

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Size:5x 5 mL Columns

historical overview: [266]) and an adequate range of specific antagonists, therefore, took time and effort to find (reviews: [303, 309])