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Consequence: the mass measured by spectrometry for the salt differs from the mass of the free peptide by the contribution of the counterion, and the physicochemical properties (stability, solubility, hygroscopicity) depend on the type of salt
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However, due to the high levels of autofluorescence in the cap1Δ/Δ background strain and the relatively low levels of Gpx3 production across all strains, we carried out shorter term assays in which cells were treated with t-BHP for 30 m which resulted in less autofluorescence in the cap1Δ/Δ strain than the 60 m treatment (Fig 4E)
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