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Experiment Condiitons Cell Line: HeLa cells Pretreatment: DMEM (cystine-free, serum-free), 37℃, 5 min Uptake conditions：0.5 mmol/l sulfasalazine or 2 μmol/l erastin / Cystine Analog / DMEM (cystine-free, serum-free), 37℃, 30 min Instrument: Fluorescent Plate Reader Filter: Ex=485 nm, Em=535 nm Application Data 2: Comparison with Radio Isotope Method Cystine uptake assay, which used to require RI measurement, can now be easily measured with a plate reader