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2.8 Pull-down experiments Chloroplast extracts (100 µg of Chl) were solubilized with 1% (w/v) dodecyl maltoside (DM) for 10 min at 4°C, and incubated with 50 μg of either purified His-TRX-f1, His-TRX-m1 and His-NTRC as bait proteins in binding buffer (BF, 25 mM Tris-HCl [pH 7.8], 150 mM NaCl, 5 mM MgCl 2 , 10% [v/v], glycerol, and cOmplete protease inhibitor [Roche]) overnight at 4°C and 45 rpm

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doi: 10.1155/2016/4134053