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doi: 10.13863/j.issn1001-4454.2016.04.040 94 ZhangCCGuWLWuXMLiYMChenCXHuangXY
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Briefly, cells fixed with 4% paraformaldehyde were blocked with 10% goat serum and 0.1% Triton-X in phosphate-buffered saline (PBS) at room temperature for 45 min, and thereafter incubated with primary antibody overnight at 4 °C
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