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For negative-mode acquisition, mobile phase A was 100% LC–MS-grade H 2 O with 10 mM ammonium acetate, 0.1% ammonium hydroxide and 0.1% medronic acid (Agilent)
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Size:2mg
Wipe the rubber septum with a 70% isopropyl alcohol swab and allow it to dry for 30 seconds before each insertion
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