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They are an important component of the neurovascular unit and are thought to make significant contributions to blood–brain barrier functions (Abbott et al., in vitro blood–brain barrier models were first being developed it was found that the presence of either cultured astrocytes or conditioned medium from cultured astrocytes produced cells with more complex tight junctions (Tao-Cheng et al., 1987) and this was essential for the preparation of cerebral endothelial monolayers with high transendothelial resistance (Dehouck et al., 1991)
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